A high-throughput screening system for G-protein-coupled receptors using β-lactamase enzyme complementation technology
نویسندگان
چکیده
منابع مشابه
Cell-based high-throughput screening assay system for monitoring G protein-coupled receptor activation using beta-galactosidase enzyme complementation technology.
A novel cell-based functional assay to directly monitor G protein-coupled receptor (GPCR) activation in a high-throughput format, based on a common GPCR regulation mechanism, the interaction between beta-arrestin and ligand-activated GPCR, is described. A protein-protein interaction technology, the InteraX trade mark system, uses a pair of inactive beta-galactosidase (beta-gal) deletion mutants...
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ژورنال
عنوان ژورنال: Acta Pharmacologica Sinica
سال: 2010
ISSN: 1671-4083,1745-7254
DOI: 10.1038/aps.2010.154